Induction of a chromatin boundary in vivo upon insertion of a TAD border

Proposed mechanistic model of Btg1 expression changes.

In mammals, the genome is spatially segmented in three-dimensional domains called TADs, which are separated by more or less strict boundaries. This organization seems to be important to properly implement gene regulation through the action of long-distance enhancers. Nevertheless, genome-wide studies on this relationship are not easy to resolve, and the relevance of each TAD boundary often needs to be taken on a case-by-case basis.

Andréa Willemin and Lucille Lopez-Delisle, from the laboratories of Denis Duboule, showed that a TAD boundary, when randomly inserted in a different chromosome, retained its ability to reshape the chromatin landscape and disturb gene expression.

This work co-supervised by Eddie Rodríguez-Carballo, led Andréa Willemin to obtain the Prix Arditi for the best Master Thesis in Biology in 2020.

The article was published in PLoS Genetics on July 22nd, 2021.